| 质粒名称: | pcDNA4 HisMax-V5-GFP-RRBP1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92150 |
| 存储实验室: | Alice Ting |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA4 HisMax |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5300 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | RRBP1,p180 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4620 bp |
| 基因突变: | In RRBP1: N951S and T1329L. E1279 is also missing. |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
钙网蛋白信号肽（插入物的 N 端）
V5（插入件上的 N 端子）
EGFP（A207K 突变）（N 端插入）
|
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMVf |
| 3' 测序引物 | BGHr |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA4 HisMax-V5-GFP-RRBP1 was a gift from Alice Ting (Addgene plasmid # 92150 ; http://n2t.net/addgene:92150 ; RRID:Addgene_92150) |
| 参考文献部分: | Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation. Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA, Ting AY. Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463. 10.7554/eLife.24463 PubMed 28441135 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA4 HisMax-V5-GFP-RRBP1 10411 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide
repeats) in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS pcDNA4 HisMax-V5-GFP-RRBP1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10411)
AUTHORS Hung V, Lam SS, Udeshi ND, Svinkina T, Guzman G, Mootha VK, Carr SA,
Ting AY
TITLE Proteomic mapping of cytosol-facing outer mitochondrial and ER
membranes in living human cells by proximity biotinylation.
JOURNAL Elife. 2017 Apr 25;6. pii: e24463. doi: 10.7554/eLife.24463.
PUBMED 28441135
REFERENCE 2 (bases 1 to 10411)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10411
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 961..1002
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 1012..1728
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1057..1078)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1318..1337)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1665..1686
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(6419..6436)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6425..6649
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6695..7123
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6782..6801)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6992..7013
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7132..7152)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7137..7466
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7317..7452
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7379..7398
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 7514..7561
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 7580..7954
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 8084..8205
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8121..8140)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8175..8194
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8254..8270)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8254..8270)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8267..8289)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8278..8294
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8302..8332)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8347..8368
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8485..8502)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8656..9244)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8736..8755)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9415..10275)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10038..10057
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10276..10380)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgctgctat ccgtgccgtt gctgctcggc ctcctcggcc tggccgtcgc ccgggatcct
961 ggcaagccga tccctaaccc tctgctgggc ctggacagca ctacggatcc aatggtgagc
1021 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
1081 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
1141 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
1201 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
1261 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
1321 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
1381 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
1441 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
1501 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
1561 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
1621 acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
1681 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggccggact
1741 cagatccgaa agcaagccag gatggatatt tacgacactc aaaccttggg ggttgtggtc
1801 tttggaggat tcatggttgt ttctgccatt ggcatcttcc tggtgtcgac tttctccatg
1861 aaggaaacgt catatgaaga agccctagcc aaccagcgca aggagatggc gaaaactcac
1921 caccagaaag tcgagaagaa aaagaaggag aaaacagtgg agaagaaagg aaagaccaag
1981 aaaaaggaag agaaacctaa tgggaagata cctgatcatg atccagcccc caatgtgact
2041 gtcctccttc gagaaccagt gcgggctcct gctgtggctg tggctccaac cccagtgcag
2101 ccccccatta tcgttgctcc tgtcgccaca gttccggcca tgccccagga gaagctggcc
2161 tcctccccca aggacaaaaa gaagaaggag aaaaaagtgg caaaagtgga accagctgtc
2221 agctctgtag tgaattccat ccaggttctc acttcgaagg ctgccatctt ggaaactgct
2281 cccaaggagg tgccgatggt ggtggtgccc ccagtgggtg ccaagggcaa cacaccagcc
2341 actggcacta ctcagggcaa aaaggcggag gggactcaga atcaaagcaa aaaggctgaa
2401 ggagccccaa accagggcag aaaggcagag ggaaccccaa accagggcaa aaagacagag
2461 ggaaccccaa accaagggaa aaaggcagag ggaaccccaa accaaggcaa aaaggcagaa
2521 ggaaccccaa accaaggcaa aaaggcggag ggggcccaga accagggtaa aaaggtagat
2581 acaaccccaa accaggggaa aaaggtggag ggggccccaa cccagggcag aaaggccgag
2641 ggggctcaga accaggccaa aaaggtagaa ggggcccaga accagggcaa aaaggcagag
2701 ggggcccaga atcagggcaa aaagggagag ggggcccaga accagggcag gaaggccgag
2761 ggggcccaga accagggcag gaaggccgag ggggcccaga atcagggcaa gaaggccgag
2821 ggggcccaga atcagggcaa gaaggccgag ggggcccaga atcagggcaa gaaggctgag
2881 ggggctcaga accagggcaa aaaggccgag ggggctcaga accagggcaa aaaagtagaa
2941 ggggcccaga accagggcaa gaaggctgag ggtgcccaga accagggcaa aaaggccgag
3001 ggggcccaga atcagggcaa aaaggccgag ggggcccaga accagggcaa gaaggcagag
3061 ggggcccaga accagggcaa gaaggccgag ggggcccaga accaggacaa gaaggccgag
3121 ggggcccaga accagggcag gaaggccgag ggggcccaga accagggcag gaaggccgag
3181 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
3241 ggggccccga accagggcaa gaaggccgag gggaccccga accagggcaa gaaggccgag
3301 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
3361 gggaccccga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
3421 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
3481 ggggcccaga accagggcaa gaaggccgag ggggcccaga accagggcaa gaaggccgag
3541 ggtgctcaga accagggcaa aaaagtagaa ggggcccaga accagggcaa gaaggccgag
3601 ggggcccaga accagggcaa gaaggccgag ggggctcaga accagggcaa aaaggccgag
3661 ggagcccaga accagggcca aaaaggagag ggagcccaga atcagggtaa aaagacagaa
3721 ggggctcagg gcaaaaaagt agaaggggcc cagaaccagg gcaagaaggc cgagggggcc
3781 cagaaccagg gcaaaaaggc agattcggtt gctaatcagg gcacaaaggt agagggtatt
3841 acaaaccagg ggaaaaaagc agaagggtcc cccagtgaag gcaaaaaggc agaagggtcc
3901 cccaaccaag gcaaaaaggc agacgcagct gccaatcagg gtaaaaagac agagtcagct
3961 tctgtccagg gcagaaatac agatgtggcc cagagcccag aggcaccaaa gcaagaggct
4021 cctgccaaga agaagtctgg ttcaaagaaa aaaggtgagc ctgggccccc agatgccgac
4081 ggccctctct acctccccta caagacgctg gtctccacgg ttgggagcat ggtgttcaac
4141 gagggcgagg cccagcggct catcgagatc ctgtctgaga aggctggcat cattcaggac
4201 acctggcaca aggccactca gaagggtgac cctgtggcga ttctgaaacg ccagctggaa
4261 gagaaggaaa aactgctggc cacagaacag gaagatgcgg ctgtcgccaa gagcaaactg
4321 agggagctca acaaggagat ggcagcagaa aaggccaaag cagcagccgg ggaggccaaa
4381 gtgaaaaagc agctggtggc ccgggagcag gagatcacgg ctgtgcaggc acgcatgcag
4441 gccagctacc gggagcacgt gaaggaggtg cagcagctgc agggcaagat ccggactctt
4501 caggagcagc tggagaatgg ccccaacacg cagctggccc gcctgcagca ggagaactcc
4561 atcctgcggg atgccttgaa ccaggccacg agccaggtgg agagcaagca gagcgcagag
4621 ctggccaagc ttcggcagga gctcagcaag gtcagcaaag agctggtgga gaagtcagag
4681 gctgtgcggc aagatgagca gcagcggaaa gctctggaag ccaaggcagc tgccttcgag
4741 aagcaggtcc tgcagctgca ggcgtcccac agggagagtg aggaggccct gcagaagcgc
4801 ctggacgagg tcagccggga gctgtgccac acgcagagca gccacgccag cctccgggcg
4861 gatgccgaga aggcccagga gcaacagcag cagatggccg agctgcacag caagttacag
4921 tcctccgagg cggaggtgcg cagcaaatgc gaggagctga gtggcctcca cgggcagctc
4981 caggaggcca gggcggagaa ctcccagctc acagagagaa tccgttccat tgaggccctg
5041 ctggaggcgg gccaggcgcg ggatgcccag gacgtccagg ccagccaggc ggaggctgac
5101 cagcagcaga ctcgcctcaa ggagctggag tcccaggtgt cgggtctgga gaaggaggcc
5161 atcgagctca gggaggccgt cgagcagcag aaagtgaaga acaatgacct ccgggagaag
5221 aactggaagg ccatggaggc actggccacg gccgagcagg cctgcaagga gaagctgcac
5281 tccctgaccc aggccaagga ggaatcggag aagcagctct gtctgattga ggcgcagacc
5341 atggaggccc tgctggctct gctcccagaa ctctctgtct tggcacaaca gaattacacc
5401 gagtggctgc aggatctcaa agagaaaggc cccacgctgc tgaagcaccc gccagctccc
5461 gcggagccct cctcggacct ggcctccaag ttgagggagg ccgaggagac gcagagcaca
5521 ctgcaggccg agtgtgacca gtaccgcagc atcctggcgg agacggaggg catgctcaga
5581 gacctgcaga agagcgtgga ggaggagcag gtgtggaggg ccaaggtggg cgccgcagag
5641 gaggagctcc agaagtcccg ggtcacagtg aagcatctcg aagagattgt agagaagcta
5701 aaaggagaac ttgaaagttc ggaccaggtg agggagcaca cgttgcattt ggaggcagag
5761 ctggaaaagc acatggcggc cgccagcgcc gagtgccaga actacgccaa ggaggtggca
5821 gggctgaggc aacttctcct agaatctcaa tctcagctcg atgccgccaa gagcgaagcc
5881 cagaaacaga gcgatgagct tgccctggtc aggcagcagt tgagtgaaat gaagagccac
5941 gtagaggatg gtgacatagc tggggcccca gcttcctccc cagaggcgcc cccagccgag
6001 caggaccccg ttcagctgaa gacgcagctg gagtggacag aagccatcct ggaggatgag
6061 cagacacagc ggcagaagct cacggccgag tttgaggagg ctcagacctc ggcatgtcgg
6121 ttacaagaag aattggagaa gctccgcaca gccggccccc tagagtcttc agaaacagag
6181 gaggcctcac agctgaagga gagactagaa aaagagaaga agttaacaag tgacctgggg
6241 cgcgccgcca cccgcctgca ggaattgctg aaaacaaccc aagaacaatt agcacgtgaa
6301 aaggatacag tgaaaaagct tcaagagcag ttagagaaag ctgaggacgg ctcctcaagc
6361 aaggagggca ccagcgtgtg actcgagtct agagggcccg tttaaacccg ctgatcagcc
6421 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
6481 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
6541 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
6601 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
6661 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
6721 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
6781 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
6841 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
6901 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
6961 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
7021 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
7081 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
7141 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
7201 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
7261 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
7321 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
7381 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
7441 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
7501 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
7561 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
7621 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
7681 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
7741 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
7801 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
7861 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
7921 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
7981 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
8041 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
8101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
8161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
8221 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
8281 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
8341 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
8401 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
8461 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
8521 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
8581 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
8641 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
8701 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
8761 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
8821 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
8881 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
8941 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
9001 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
9061 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
9121 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
9181 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
9241 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
9301 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
9361 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
9421 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
9481 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
9541 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
9601 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
9661 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
9721 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
9781 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
9841 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
9901 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
9961 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
10021 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
10081 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
10141 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
10201 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
10261 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
10321 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
10381 cacatttccc cgaaaagtgc cacctgacgt c
//
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